视频教程

这里展示了一系列PEAKS的视频教程,如果您觉得这里的教程资源对你有帮助,欢迎关注我们的微信公众号。

PEAKS Overview

For a quick and easy overview of what PEAKS has to offer, this video is a great place to start. It covers de novo sequencing, database searching, including automated summary reporting, de novo only findings, as well as the additional features of homology searching, multi-engine reporting and quantification.(5 minutes)

PEAKS Overview Video

Precursor Ion Quantification (SILAC)

PEAKS provides more accurate and reliable SILAC quantification results by incorporating enhanced computational features. (7.5 minutes)

Precursor Ion Quantification (SILAC)

PEAKS Q – Isobaric Labeling, TMT/iTRAQ

Protein/peptide quantification with TMT/iTRAQ offering:
• Accuracy & Sensitivity
• Large-scale study with labelling quantification
(9 minutes)

PEAKS Q - Isobaric Labeling, TMT-iTRAQ

Peptide Feature Intensities

PEAKS now incorporates quantitative information into your identification results. Our quantitative module has accurate and sensitive peptide feature detection that can be used to get the relative abundance of a peptide. By matching the peptide feature area to an identified MSMS, you can:
• Determine the most abundant peptides in your sample
• Obtain quantitative information on endogenous peptides
(3 minutes)

Peptide Feature Intensities - PEAKS

Multi-Round Search

Often there are many spectra remaining after a database search that have promising de novo sequences, leaving you wondering what the source of these spectra are. We call these ‘de novo only spectra’. Multi-Round search gives you the ability to search only these spectra. This helps you:
• Remove spectra produced from contaminants
• Find endogenous peptides in a enzyme digested sample
• Refine search parameters to identify difficult targets
(3.5 minutes)

PEAKS Multi-Round Search

PTM Profiling

Detect and quantify modifications with LC-MS/MS data and compare PTM profiling on proteins between samples. (3.5 minutes)

PEAKS PTM Profiling

Peptide De Novo Sequencing

Check out this video for a more in-depth analysis on PEAKS’ peptide de novo sequencing. This video will give a brief overview of how peptide de novo sequencing can be useful, as well as give a demonstration on how to perform a de novo analysis using PEAKS. (5 minutes)

PEAKS Peptide De Novo Sequencing

PEAKS Q: Label Free Quantification

In addition to protein and peptide identification, PEAKS excels at accurate label free quantification. This video predominantly uses slides to illustrate the fundamentals of the method. (5 minutes)

PEAKS Q- Label Free Quantification

PEAKS Q: Label Free Quantification – Live Walkthrough

This video skips ahead to the question of “How do I analyze a set of data using PEAKS?”. Basics of the software are addressed quickly as to maximize attention towards result explanation and value. We even explain how to use PEAKS label free quantification to identify peptide features without database peptide identification. (6 minutes)

PEAKS Q - Label Free Quantification Live Walkthrough